carbohydrate detection and lectin isolation from tegumental tissue of fasciola hepatica

background: fascioliasis is a chronic hepatic disease and may be resulted from mechani­cal/molecular parasite adhesion to host liver tissue. the aim of this study was to detect surface car­bohydrate and lectin, carbohydrate-binding protein isolation that might be responsible of this molecular binding. methods: the present experimental work was conducted in the department of medical parasitol­ogy and mycology, school of public health, tehran university of medical sciences, te­hran, iran.  fasciola hepatica parasites were collected from abattoir (saman, tehran, iran) and surface mannose-carbohydrate was detected by fluorescein isothiocyanate (fitc) conju­gated lectin (lentil). lectin of tegumental tissue from f. hepatica was isolated by affinity chroma­tography and detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). results: mannose carbohydrate was observed on the surface of tegumental tissue from para­site under fluorescence microscope. carbohydrate-binding protein or lectin with mw of 50 kda also was isolated from homogenized tegument of helminth. conclusion: these results are important for understanding of molecular pathogenesis of f. hepat­ica at the chronic phase of fascioliasis